Využití průtokové cytometrie pro sledování proliferace a diferenciace monoblastů transformovaných onkogenem v-myb
| Title in English | Use of flow cytometry for exploring proliferation and differentiation of v-myb-transformed monoblasts |
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| Authors | |
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | Analytická cytometrie III |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | p53; myb; differentiation; proliferation; apoptosis; flow cytometry |
| Description | The aim of this work was to determine whether transforming function of the v-myb oncogene can be suppressed by human p53. Therefore, BM2 monoblasts were transfected with cDNA coding for human p53 and the role of this protein in BM2 cells was addressed. p53 protein induced growth arrest in G2 phase of the cell cycle. At the same time, BM2 cells expressing p53 resumed differentiation capacity as documented by increased cell adherence, phagocytic activity, oxygen radical formation and alpha-naphtylactetate esterase activity. In addition, intracellular pH was decreased and amount of cell surface CD11b integrin was up-regulated in these cells. These results document that p53 induces maturing of BM2 monoblasts to monocytes. Once p53-expressing cells are treated with retinoic acid, they undergo maturing to terminal stage of macrophages. Our results show that p53 tumor suppressor is dominant over v-Myb oncoprotein: it changes v-Myb activity in transformed cells and resumes their natural differentiation capability. |
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