Characterisation of Bifidobacterium and Lactobacillus strains in infant faeces samples

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Authors	ŠPANOVÁ Alena RITTICH Bohuslav KLESNILOVÁ Lenka ŠKAPOVÁ Tereza
Year of publication	2005
Type	Article in Proceedings
Conference	BioMicroWorld-2005
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	Bifidobacterium; Lactobacillus; infant faeces; PCR; ARDRA; RAPD
Description	In the present study genus-specific and species-specific PCR primers (targeted on a highly conserved 16S rDNA region) were used to identify Bifidobacterium and Lactobacillus strains isolated from infant faecal samples. Bifidobacteria were differentiated at the species level by a restriction fragment length polymorphism analysis using the enzymes BamHI, Sau3AI, and TaqI (amplified ribosomal DNA restriction analysis ARDRA). Bifidobacteria and lactobacilli were also differentiated using RAPD analysis. Altogether 40 strains of Bifidobacteria and 20 strains of Lactobacillus identified in the collected infant faeces were analysed.
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