Programovaná buněčná smrt v-myb transformovaných monoblastů je nezávislá na kaspázách
| Title in English | Programmed cell death of v-myb-transformed monoblasts is caspase-independent |
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| Authors | |
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Chemické listy |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | programmed cell death; autophagy; v-myb |
| Description | We studied programmed cell death (PCD) in two promonocytic cell lines - human U937 cells and chicken v-myb-transformed monoblasts. PCD was induced using three cytotoxic agents: camptothecin, cycloheximid and arsenic trioxide. Cell death was analyzed by measurement of mitochondrial membrane potential, DNA fragmentation, F-actin depolymerization and caspase activity. We found that camptothecin-treated BM2 cells undergo caspase-independent cell death while similarly treated U937 cells undergo caspase-dependent PCD. This suggests that v-Myb that is constitutively expressed in BM2 cells may prevent caspases from activation thus inducing alternative caspase-independent cell death pathways. |
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