Identifikace bifidobaktérií využívaných v mlékárenském průmyslu pomocí polymerázové řetězové reakce a restrikční analýzy amplfikované ribozomální DNA
| Title in English | Identification of bifidobacteria used to ferment dairy products by polymerase chain reaction and amplified ribosomal DNA restriction analysis |
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| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | VIII. Pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | Bifidobacterium; PCR; Restriction analysis; |
| Description | Bifidobacterial strains from the collection of dairy cultures Laktoflora (Prague, Czechrepublic)were identificated by molecular biological metods.Genus specific PCR with primers Pbi F1 and Pbi R2 and species specific PCR were used. Genus specific PCR product 914 bp was restricted by enzymes Bam HI,Sau3 AI and TaqI (ARDRA).The ARDRA method and genus and species specific PCR method are available for the differentiation and identification of some species of bifidibacteria. |
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