Testování nosičů aktivovaných ionty lanthanoidů při štěpení plazmidové DNA
Title in English | Testing of carriers activated with lanthanide ions in cleavage of plasmid DNA |
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Authors | |
Year of publication | 2004 |
Type | Article in Proceedings |
Conference | VIII. Pracovní setkání biochemiků a molekulárních biologů |
MU Faculty or unit | |
Citation | |
Field | Microbiology, virology |
Keywords | Carriers; Lanthanide ions; Cleavage; DNA; plasmid pBR322 |
Description | Nonmagnetic carriers activated by lanthanide ions were used for cleavage of plasmid DNA pBR322. Following carriers were used: methacrylate copolymer covered by EDTA groups and Chelex 100 - cross-linked polystyrene beads with IDA groups (iminodiacetic acid). Carriers were activated by lanthanide ions: Eu3+, La3+, Nd3+, Pr3+ a Gd3+. |
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