Examination of membrane protein expression in Paracoccus denitrificans by two-dimensional gel electrophoresis
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Basic Microbiology |
| MU Faculty or unit | |
| Citation | |
| Web | Publikace v pdf formátu |
| Field | Biochemistry |
| Keywords | Paracoccus denitrificans; proteome; two-dimensional gel electrophoresis |
| Description | The well-known metabolic versatility of the soil bacterium Paracoccus denitrificans poses a challenge for the modern proteomic approaches. We describe here the improved conditions that allow good separations and quantitative analyses of hundreds of membrane or periplasmic proteins. As an example, the results of a screening for membrane proteins associated predominantly with aerobic or anaerobic (denitrifying) modes of growth are presented. |
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