Testování vlivu magnetických nosičů na bázi celulózy na průběh PCR
| Title in English | Testing of cellulose based magnetic carriers on the PCR course |
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| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | VIII. Pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | Cellulose; magnetic carriers; Bifidobacterium; PCR |
| Description | Influence of 8 magnetic carriers, including components used in their synthesis, on the PCR course was investigated. PCR primers Pbi F1 and Pbi R2 specific to genus Bifidobacterium were used. Specific PCR product (914 bp) was detected using agarose gel electrophoresis. Results of PCR were compared with positive controls. DNA of four different concentrations isolated from Bifidobacterium cells was used as positive control(it was added to PCR mixture without magnetic carriers). |
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