Polypodium x mantoniae and new localities of P. interjectum in the Czech Republic confirmed using flow cytometry
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Periodical |
| Magazine / Source | Preslia |
| MU Faculty or unit | |
| Citation | |
| web | http://www.preslia.cz/P034CBur.pdf |
| Field | Botany |
| Keywords | AT frequence;C-value;ferns;flow cytometry;genome size;nuclear DNA content;PI/DAPI index;Polypodiaceae;Pteridophyta |
| Description | Flow cytometry measurements confirmed the occurrence of Polypodium x mantoniae (P. interjectum x P. vulgare) in three localities from the eastern part of the Czech Republic (Blansko and Rudice North of Brno and Javoříčko WNW of Olomouc). Nuclear DNA contents (+/- Sx) were determined for P. vulgare (2C=29.00 +/- 0.32 pg), P. x mantoniae (2C=37.18 +/- 0.38 pg) and P. interjectum (2C=45.24 +/- 0.31 pg) by a PAS Partec GmbH flow cytometer (PI staining / standard Vicia faba, 2C=26.9 pg). The relative DNA content ratio was measured between all pairs of taxa (plus minus Sx range) P. vulgare : P. x mantoniae = 0.746 plus minus 0.005; P. vulgare : P. interjectum = 0.595 plus minus 0.001; P. x mantoniae : P. vulgare = 1.340 plus minus 0.008; P. x mantoniae : P. interjectum = 0.797 plus minus 0.005; P. interjectum : P. vulgare = 1.681 plus minus 0.003; P. interjectum : P. x mantoniae = 1.255 plus minus 0.008. Six new localities of Polypodium interjectum were found in the region of Moravský Kras (= Moravian Karst, north of Brno). From the PI/DAPI index it can be inferred that the AT/GC ratio (or heterochromatin occurrence) is 1.05x bigger in P. x mantoniae than in P. vulgare and 1.08x bigger in P. interjectum than in P. vulgare. Anatomical data (number of thick-walled cells of the anulus, spore length and stomata length) of selected specimens and live samples from the Czech Republic were in good agreement with the range of variation of these features published by earlier authors from other European countries. A brief historical survey of knowledge of P. interjectum in the Czech Republic is included. |
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