Charakterizace dvou myb-like proteinů z Arabidopsis thaliana, které vykazují afinitu k telomerickým sekvencím
| Title in English | Characterisation of two Arabidopsis thaliana myb-like proteins showing affinity to telomeric DNA sequence |
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| Authors | |
| Year of publication | 2003 |
| Type | Article in Proceedings |
| Conference | VII. Pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | myb-like proteins; Arabidopsis thaliana |
| Description | Using a genomic approach, two Arabidopsis thaliana genes coding for candidate myb-like telomere bnding proteins were cloned and expressed in E. coli. Both proteins, termed as AtTBP2 and AtTBP3, contain a single Myb-like DNA-binding domain at the N-terminus, and a histone H1/H5-like DNA-binding domain in the middle of the protein sequence. Gel-retardation assays showed that each of the two proteins is able to bind G-rich strand and double-strand DNA of plant telomeric sequence with the affinity proportional to a number of telomeric repeats. |
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