A Five-year Study of the Presence of Borrelia and Antibodies to Borrelia in Small Rodents

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Authors

VOSTAL Karel ŽÁKOVSKÁ Alena NEČASOVÁ Marcela

Year of publication 2003
Type Article in Proceedings
Conference Current research in Zoonotic Ecology and Epidemiology
MU Faculty or unit

Faculty of Science

Citation
Field Immunology
Keywords Borrelia burgdorferi sensu lato; ELISA; IgG; rodents; PCR-RFLP; SDS-PAGGE
Description Testing of wild-living rodents for the presence of antibodies against Borrelia burgdorferi sensu lato using indirect ELISA method. Examination for the presence of spirochaetes in rodent tissues by dark- field microscopy. Isolation of samples and identification of them by PCR-RFLP method. Confirmation of results by SDS-PAGGE method.
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