Study of the gene expression in malignant diseases by cDNA microarray technology
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Proceedings |
| Conference | Biophysics of the Genome |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | human genome structure |
| Description | At the present time, a number of various diseases increases and thereby also increases the necessity of new therapeutic methods and new approaches to research. In last decade, many new technologies have been evolving, among others the technology called cDNA microarrays. This technology enables the detection of global gene-expression profiles, the identification of new molecular cancer markers. It makes possible the comparison of gene-expression profiles of various cancer tumors and cell lines under different environmental stress conditions. cDNA microarrays consist of hundreds or thousands spots with different cDNA sequences (resp. oligonucleotide sequence strands) organized in grids and they can contain more than 20,000 specific sequences on a single slide. This technology is based on process of hybridization between immobilized cDNAs (oligonucleotides sequences) and reverse-transcribed cDNA from sample of total RNA. We used cDNA microarray technology to study gene expression profiles of colon cancer tissue derived from patients with colorectal carcinoma. Hereditary forms of colorectal carcinoma are often associated with familial polyposis syndromes (FAP) or hereditary nonpolyposis colorectal cancer (HNPCC). The mutations responsible for these syndromes are the mutations of APC gene (FAP) and DNA mismatch repair genes e.g. hMSH2, hMLH1 (HNPCC). Both pathways can be discerned in sporadic cases, but also many remaining genes can be deregulated. RNA, acquired from patients, was reverse-transcribed in the presence of fluorescent-labeled dCTP and was hybridized on the glass cDNA microarray. We identified genes, which were up- or down-regulated in tumor samples. In the next research we will study the topological organization of these genes in the nucleus by FISH technique. |
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