Identifikace ekotypů lipnice luční pomocí izoenzymového systému EST

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Title in English Identification of Kentucky bluegrass (Poa pratensis) ecotypes and varieties by EST isozymes
Authors

JANEČEK Josef ČERNOCH Vladimír STONEBERG HOLT Sierra Dawn HOROVÁ Lucie BOTH Zdeněk

Year of publication 2002
Type Article in Proceedings
Conference Využití molekulárních markerů v biologii, šlechtění a uchovávání genových zdrojů rostlin
MU Faculty or unit

Faculty of Science

Citation
Field Botany
Keywords PAGE electrophoresis; Kentucky bluegrass; esterase isozymes; Poa pratensis
Description Kentucky bluegrass (Poa pratensis,L.) ecotypes from the USA and Europe and from varieties Slezanka, Bohemie, Moravanka and Harmonie developed at the Plant Breeding Station Hladké Životice were analysed by native PAGE electrophoresis for banding patterns of esterase isozymes (EC 3.1.1.1.). A variety of preparation methods (employing different types of extract buffers, electrophoresis, staining, gel processing and evaluation) was used to select the best procedure for ecotype and variety identification using EST isozymes.
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