Identifikace ekotypů lipnice luční pomocí izoenzymového systému EST

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Title in English	Identification of Kentucky bluegrass (Poa pratensis) ecotypes and varieties by EST isozymes
Authors	JANEČEK Josef ČERNOCH Vladimír STONEBERG HOLT Sierra Dawn HOROVÁ Lucie BOTH Zdeněk
Year of publication	2002
Type	Article in Proceedings
Conference	Využití molekulárních markerů v biologii, šlechtění a uchovávání genových zdrojů rostlin
MU Faculty or unit	Faculty of Science
Citation
Field	Botany
Keywords	PAGE electrophoresis; Kentucky bluegrass; esterase isozymes; Poa pratensis
Description	Kentucky bluegrass (Poa pratensis,L.) ecotypes from the USA and Europe and from varieties Slezanka, Bohemie, Moravanka and Harmonie developed at the Plant Breeding Station Hladké Životice were analysed by native PAGE electrophoresis for banding patterns of esterase isozymes (EC 3.1.1.1.). A variety of preparation methods (employing different types of extract buffers, electrophoresis, staining, gel processing and evaluation) was used to select the best procedure for ecotype and variety identification using EST isozymes.
Related projects:	Spatiotemporal biodiversity dynamics in ecosystems of Central Europe