Analýza proteomu bakterie Paracoccus denitrificans

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Title in English Analysis of proteome of bacterium Paracoccus denitrificans
Authors

BOUCHAL Pavel PŘECECHTĚLOVÁ Petra ZDRÁHAL Zbyněk KUČERA Igor

Year of publication 2003
Type Article in Proceedings
Conference VII. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Proteome; Paracoccus denitrificans; Membrane proteins; Two-dimensional electrophoresis; Mass spectrometry
Description Gramnegative bacterium Paracoccus denitrificans is able to grow under broad variety of conditions. Adaptation to altered conditions strongly depends on changes in expression of metabolically active proteins. For study on changes of protein levels in whole-cell extract as well as in bacterial membrane of Paracoccus denitrificans, we have developed methods based on two-dimensional electrophoresis with immobilised pH gradients. Optimization of critical points of both protocols is demonstrated. We have compared effect of anaerobiosis on protein composition of bacterial membrane using optimized method. Several proteins on protein maps were sucessfully identified using MALDI-TOF mass spectrometry.
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