Determination of optimal conditions for detection of acute myeloid leukemia t(18;21) and t(15;17) translocations using RT-PCR

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Authors	FIALOVÁ Markéta ŠEVČÍKOVÁ Sabina ŠMARDA Jan MICHÁLEK Jaroslav
Year of publication	2002
Type	Article in Periodical
Magazine / Source	Neoplasma
MU Faculty or unit	Faculty of Science
Citation
Field	Oncology and hematology
Keywords	RT-PCR; AML; translocation
Description	Prognosis of patients with acute myeloid leukemia (AML) is not satisfactory. The aim of the study is to improve diagnosis of MAL in cases characterized by specific chromosomal translocations t(18;21) and t(15;17) using RT-PCR. To determine optimal conditions for detection of both translocations we performed a detail study of experimental onditions for RT-PCR on human cell lines NB-4 and KASUMI-1. The results proved to be valid for clinical diagnstics of the translocations in blood and/or bone marrow samples of AML patients.
Related projects:	Detection and monitoring of certain chromosomal abnormalities in children suffering from acuta myeloid leukemia