Degradation of plasmid and high-molecular weight DNAs by lanthanide ions

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Authors

ŠPANOVÁ Alena RITTICH Bohuslav FALK Martin

Year of publication 2002
Type Article in Proceedings
Conference 22nd International Symposium on the Separation of Proteins, Peptides and Polynucleotides
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Lanthanide ions; degradation; DNAs
Description The effect of La3+,Eu3+,Pr3+,Gd3+ and Nd3+ chlorides on the cleavage of plasmid pBR322 and high-molecular weight calf thymus DNA was reported. Cleavage was followed spectrophotometrically and using agarose gel electrophoresis. In the case of plasmid pBR322 DNA the formation of linear and nicked plasmid forms was investigated depending on reaction conditions.
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