Advances in high-resolution cytometry of FISH dots in interphase cell nuclei

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Informatics. Official publication website can be found on muni.cz.

Authors	KOZUBEK Michal KOZUBEK Stanislav LUKÁŠOVÁ Emilie BÁRTOVÁ Eva SKALNÍKOVÁ Magdalena MATULA Pavel MATULA Petr JIRSOVÁ Pavla GAŇOVÁ Alena KOUTNÁ Irena
Year of publication	2000
Type	Conference abstract
MU Faculty or unit	Faculty of Informatics
Citation
Description	Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D analysis up to high-resolution confocal 3-D studies.
Related projects:	Application of computer image analysis in optical microscopy