Transition between two forms of heterochromatin at plant subtelomeres
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | Chromosome Research |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | chromatin structure; nucleosome positioning; plant telomeres and subtelomeres |
| Description | We have analysed the chromatin structure of two subtelomeric tandem repeats from the plant Silene latifolia: X43.1 and 15Ssp. X43.1 shows two distinct nucleosome periodicities - 157 and 188bp. Preferred positions of its two nucleosomes have been mapped at both low and high resolution and the experimental results correspond to computer-redicted positions. 15Ssp is a newly-discovered sequence showing a telomere-associated position by PCR and a subtelomeric location by pulsed-field gel electrophoresis and fluorescence in situ hybridisation. Its 159 bp sequence unit shows a tandem arrangement and the presence of micrococcal nuclease-hypersensitive sites. Use of a chemical nuclease results in a regular nucleosome ladder of 157 bp periodicity. 15Ssp mononucleosomes show instability and absence of specific positioning, features typical for telomeric chromatin. |
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