Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae)
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | Parasitology |
| MU Faculty or unit | |
| Citation | |
| Field | Zoology |
| Keywords | Monogenea; Diplozoidae; PCR; ITS2; RFLP; genetic distance |
| Description | The second internal transcribed spacer (ITS2)of the ribosomal RNA gene array of 6 species of diplozoids was amplified by PCR and sequenced. These sequences clearly demonstrate discrimination at the species level and confirm the validity of species determined by morphological identification. No intraspecific variation was found in the ITS2 sequences. There were no differences in the ITS2 sequenced of P. homoion from populations parasitizing different host species. The length of the PCR product allowed discrimination of E. nipponicum from the Paradiplozoon species. Digestion of the amplified ITS2 fragment with enzymes AluI, HaeIII and HifI provided useful genetic markers for species identification. This represent the first molecular taxonomic study of these interesting parasites and demonstrates the utility of these methods for addressing questions of systematics. |
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