Evaluation of hydroxyl radical-scavenging activity of baicalin using HPLC method

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Authors

PAULOVÁ Hana BOCHOŘÁKOVÁ Hana SLANINA Jiří TÁBORSKÁ Eva

Year of publication 2000
Type Article in Periodical
Magazine / Source Pharm.Pharmacol.Lett.2000
MU Faculty or unit

Faculty of Medicine

Citation
Field Biochemistry
Keywords baicalin; hydroxyl radical;
Description Abstract. The ability of baicalin, the flavonoid from the roots of the medicinal plant Scutellaria baicalensis, to scavenge hydroxyl radicals was studied with using sensitive HPLC method. The hydroxyl radicals were generated by UV photolysis of H2O2 and salicylic acid was used as a trapping agent. The hydroxylation products of salicylic acid, 2,3- and 2,5- dihydroxybenzoic acids, were detected by HPLC. Baicalin caused decrease in the production of 2,3- and 2,5 - dihydroxybenzoic acid in a concentration dependent manner and did not affect peaks of the standard of these dihydroxybenzoic acids. These results indicate that baicalin scavenges hydroxyl radicals generated by UV photolysis of H2O2. IC(50) values were estimated.
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