Evaluation of hydroxyl radical-scavenging activity of baicalin using HPLC method
| Authors | |
|---|---|
| Year of publication | 2000 |
| Type | Article in Periodical |
| Magazine / Source | Pharm.Pharmacol.Lett.2000 |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | baicalin; hydroxyl radical; |
| Description | Abstract. The ability of baicalin, the flavonoid from the roots of the medicinal plant Scutellaria baicalensis, to scavenge hydroxyl radicals was studied with using sensitive HPLC method. The hydroxyl radicals were generated by UV photolysis of H2O2 and salicylic acid was used as a trapping agent. The hydroxylation products of salicylic acid, 2,3- and 2,5- dihydroxybenzoic acids, were detected by HPLC. Baicalin caused decrease in the production of 2,3- and 2,5 - dihydroxybenzoic acid in a concentration dependent manner and did not affect peaks of the standard of these dihydroxybenzoic acids. These results indicate that baicalin scavenges hydroxyl radicals generated by UV photolysis of H2O2. IC(50) values were estimated. |
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