Efekt farmaceutických konzervantů na antibakteriální účinek bakteriofágů proti Pseudomonas aeruginosa a Staphylococcus aureus
| Title in English | Effect of pharmaceutical preservatives on the antibacterial activity of bacteriophages against Pseudomonas aeruginosa and Staphylococcus aureus |
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| Authors | |
| Year of publication | 2024 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Due to frequent antibiotic resistance, infections caused by pathogenic strains of Pseudomonas aeruginosa and Staphylococcus aureus represent a significant threat to human health. Therefore, it is necessary to find an alternative to antibiotic treatment, which can be phage therapy, i.e. the application of bacterial viruses, so-called phages, in the treatment of bacterial infections. To implement it in practice, it is necessary to process the phages into a pharmaceutical form with regard to the protein nature of the phage particles so as to preserve the stability of their antibacterial effect. This is the subject of our study. Using the plaque method, we determined the stability of the number of infectious particles in staphylococcal phage of the Kayvirus genus and pseudomonad phage of the Pbunavirus genus. Phages with a titer of 10^9 PFU/mL were stored for 13 weeks in the form of purified products in physiological saline with twelve pharmaceutical preservatives that are used in a number of pharmaceutical forms. The preservative was recognized as suitable for phage preparations if the phage titer did not fall below 10^7 PFU/mL and no other incompatibilities (e.g., formation of a precipitate) were detected. Furthermore, the stability of a cocktail of the two aforementioned phages was determined, which was processed into lyophilized maltodextrin tablets as a purificate in SM buffer with a titer of 10 8 PFU/mL and stored for six months at temperatures of 4 and 25 °C. Representatives of salts of organic acids, phenols, alcohols, and parabens were compatible with both tested phages. Processing of phages into lyophilized tablets according to the optimized procedure led to an increase in the stability of Kayvirus phage at 25 °C for three months. This solid dosage form also has a number of advantages such as simpler application, dosing, and storage. The variable stability of phages from different genera observed in this study points to the need for optimization of the final product with respect to the specific therapeutic phage. The presented results can be applied in future studies focused on the preparation of pharmaceutical phage therapy products and their long-term storage. |
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