Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells
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| Year of publication | 2024 |
| Type | Appeared in Conference without Proceedings |
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| Description | Human embryonic stem cells (hESCs) are able to differentiate into any cell type of a human body, have self-renewal abilities and very high proliferative activity1. These unique abilities enable their use in cell therapy, disease modelling, and drug development. The derivation of hESCs is usually performed using an animal or human cell feeder layer, which is undefined and potentially contagious, and because of these difficulties, there is a tendency to replace feeders with xeno-free defined substrates in recent years2.We used truncated vitronectin with E-cadherin as adefined xeno-free substrate for the derivation of hESCs for the first time, derived three hESC lines, and confirmed their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers (ectoderm, mesoderm, and endoderm)3. We confirmed for the first time that truncated vitronectin with E-cadherin is a defined xeno-free substrate that is suitable for the derivation of hESCs involved in cell therapies. |
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