Challenges of <i>in situ</i> hybridization in miRNA analysis of triple-negative breast cancer morphological diversity

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Authors	KOLECKOVA Marketa BEZDEKOVA Michala BOUCHAL Jan JANIKOVA Maria BOHOŠOVÁ Júlia SLABÝ Ondřej SVOBODA Marek SROVNAL Josef VOMACKOVA Katherine UBERALL Ivo KOLAR Zdenek
Year of publication	2023
Type	Article in Periodical
Magazine / Source	Neoplasma
MU Faculty or unit	Central European Institute of Technology
Citation
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Doi	http://dx.doi.org/10.4149/neo_2023_220519N533
Keywords	triple-negative breast cancer (TNBC); miRNA; in situ hybridization; RT-qPCR
Description	miRNA expression in triple-negative breast cancers (TNBC) has mainly been studied from a methodological viewpoint. However, it has not been considered that miRNA expression profile may be associated with a specific morphological entity inside every tumor. The verification of this hypothesis on a set of 25 TNBCs was the subject of our previous work, where we confirmed specific expression of the studied miRNAs in 82 samples of different morphologies including inflammatory infiltrate, spindle cell, clear cell, and metastases after RNA extraction and purification as well as microchip and biostatistical analysis. In the current work, we demonstrate a low suitability of in situ hybridization method for miRNA detection compared to RT-qPCR, and in detail discuss the biological role of 8 miRNAs with the most significant changes of expression.