High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis

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Authors

AHMADLI Ulkar KALIDASS Manikandan CRHÁK KHAITOVÁ Lucie FUCHS Joerg CUACOS Maria DEMIDOV Dmitri ZUO Sheng PEČINKOVÁ Jana MASCHER Martin INGOUFF Mathieu HECKMANN Stefan HOUBEN Andreas ŘÍHA Karel LERMONTOVA Inna

Year of publication 2023
Type Article in Periodical
Magazine / Source Plant Communications
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://doi.org/10.1016/j.xplc.2022.100507
Doi http://dx.doi.org/10.1016/j.xplc.2022.100507
Keywords centromere; kinetochore null 2; CENPC-k; cenh3-4; temperature stress; haploid induction
Description Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, haploid induction via mutation of the centromere-specific histone H3 (cenH3) has been shown when the mutant is outcrossed to the wild-type, and the wild-type genome remains in the haploid progeny. However, factors that affect haploid induction are still poorly understood. Here, we report that a mutant of the cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as a haploid inducer when pollinated by the wild-type. We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold. We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines, suggesting that haploid-inducing lines in crops can be identified in a naturally occurring or chemically induced mutant population, avoiding the generic modification (GM) approach at any stage. Furthermore, a cenh3-4 mutant functioned as a haploid inducer in response to short-term heat stress, even though it did not induce haploids under standard conditions. Thus, we identified KNL2 as a new target gene for the generation of haploid-inducer lines and showed that exposure of centromeric protein mutants to high temperature strongly increases their haploid induction efficiency.
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