Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium

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Authors

ČERNÍK Marek POLAKOVA Kamila KUBALA Lukas VÍTEČKOVÁ WÜNSCHOVÁ Andrea MAC GILLAVRY DANYLEVSKA Anna PEŠKOVÁ Michaela VÍTEČEK Jan

Year of publication 2023
Type Article in Periodical
Magazine / Source ACS Biomaterials Science and Engineering
MU Faculty or unit

Faculty of Science

Citation
Web https://doi.org/10.1021/acsbiomaterials.2c00887
Doi http://dx.doi.org/10.1021/acsbiomaterials.2c00887
Keywords 3D printing; endothelial cell; in vitro model; plasma oxidation; PDMS; surface modification
Description On-chip vascular microfluidic models provide a great tool to study aspects of cardiovascular diseases in vitro. To produce such models, polydimethylsiloxane (PDMS) has been the most widely used material. For biological applications, its hydrophobic surface has to be modified. The major approach has been plasma-based surface oxidation, which has been very challenging in the case of channels enclosed within a microfluidic chip. The preparation of the chip combined a 3D-printed mold with soft lithography and commonly available materials. We have introduced the high-frequency low-pressure air-plasma surface modification of seamless channels enclosed within a PDMS microfluidic chip. The plasma treatment modified the luminal surface more uniformly than in previous works. Such a setup enabled a higher degree of design freedom and a possibility of rapid prototyping. Further, plasma treatment in combination with collagen IV coating created a biomimetic surface for efficient adhesion of vascular endothelial cells as well as promoted long-term cell culture stability under flow. The cells within the channels were highly viable and showed physiological behavior, confirming the benefit of the presented surface modification.
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