Application of long-read sequencing in chronic lymphocytic leukemia cases with complex karyotype.

Investor logo

Warning

This publication doesn't include Faculty of Sports Studies. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.
Authors

ADAMOVÁ Sabina ONDROUŠKOVÁ Eva SVATOŇ Jan JAROŠOVÁ Marie BOHÚNOVÁ Michaela PÁL Karol ČERNOVSKÁ Karolína ZÁVACKÁ Kristýna PORC Jakub Paweł KOTAŠKOVÁ Jana PLEVOVÁ Karla STRÁNSKÁ Kamila

Year of publication 2023
Type Conference abstract
MU Faculty or unit

Central European Institute of Technology

Citation
Attached files
Description Complex karyotype (CK) typically involves various, often extensive numerical and structural chromosomal abnormalities. In chronic lymphocytic leukemia (CLL), it represents an established adverse prognostic marker. Common methods to detect CK include classical cytogenetics and genomic microarray, however, their resolution is limited. We aimed to explore the ability of long-read sequencing for the precise characterization of complex genomic variants in CLL patient samples. CK cases were identified and characterized using classical (IL-2/CpG-stimulated chromosomal banding) and molecular (24×Cyte Multicolor FISH, CytoScan HD Array) cytogenomics. For long-read sequencing, high molecular weight DNA was isolated using chloroform-isopropanol extraction, fragmented by needle shearing, and short DNA fragments were eliminated. The sequencing libraries were prepared using the Ligation Sequencing Kit (Oxford Nanopore Technologies) and sequenced on the MinION or PromethION platform. Reads were aligned to the hg38 human genome reference, and breakpoints were identified with the SVIM variant caller.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info