BIOIMAGING OF ELEMENTS AND PROTEINS BY LA-ICP-MS IN MEDICINE AND PHARMACEUTICAL RESEARCH

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Authors

KUCHYNKA Michaela VLČNOVSKÁ Marcela HLOŽKOVÁ Jana SCHEER Peter OPATŘILOVÁ Radka VACULOVIČOVÁ Markéta KANICKÝ Viktor MASAŘÍK Michal VACULOVIČ Tomáš

Year of publication 2022
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Science

Citation
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Description In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease. The main emphasis of this contribution is to demonstrate the unique method of laser ablation with inductively coupled plasma and mass spectrometry (LA-ICP-MS) not only as a method of element analysis, but also as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Proteins are imaged using a combination of immunohistochemical methods – binding of nanoparticles labeled antibodies to a specific protein. The main advantages of using LA-ICP-MS are not only the acquisition of complex (metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke), but also low detection limits compared to other conventionally used protein imaging techniques, or multiplex analysis of a single sample.
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