LC-HRMS based method for suspect/non-targeted screening for biomarkers of chemical exposure in human urine

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Authors

TKALEC Žiga CODLING Garry Paul KLÁNOVÁ Jana HORVAT Milena KOSJEK Tina

Year of publication 2022
Type Article in Periodical
Magazine / Source Chemosphere
MU Faculty or unit

Faculty of Science

Citation
Web https://www.sciencedirect.com/science/article/pii/S0045653522010438
Doi http://dx.doi.org/10.1016/j.chemosphere.2022.134550
Keywords Xenobiotic; Exposome; Biomonitoring; Non-targeted; Sample preparation; HBM4EU
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Description Every day we are exposed to a cocktail of anthropogenic compounds many of which are biologically active and capable of inducing negative effects. The simplest way to monitor contaminants in a population is via human biomonitoring (HBM), however conventional targeted approaches require foreknowledge of chemicals of concern, often have compound specific extractions and provide information only for those compounds. This study developed an extraction process for human biomarkers of interest (BoE) in urine that is less compound specific. Combining this with an ultra-high resolution mass spectrometer capable of operating in full scan, and a suspect and non-targeted analysis (SS/NTA) approach, this method provides a more holistic characterization of human exposure. Sample preparation development was based on enzymatically hydrolysed urine spiked with 34 native standards and extracted by solid-phase extraction (SPE). HRMS data was processed by MzMine2 and 80% of standards were identified in the final data matrix using typical NTA data processing procedures.
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