Topography of some genes and chromosomes in interphase nuclei of human cells
| Authors | |
|---|---|
| Year of publication | 1999 |
| Type | Article in Proceedings |
| Conference | Fluorescence microscopy and fluorescent probes |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | gene topography |
| Description | For the present, only limited information about the arragement of chromosomes and individual genes in interphase nuclei exists. The results presented in this work contribute to the knowledge of the location of some genes and chromosomes in interphase nuclei of different cell types. Using fluorescence in situ hybridization (FISH) combined with high resolution cytometry (HRCM) developed in our laboratory, we have investigated the distributions of the ABL, BCR, c-MYC and IGH genes and whole chromosomes 8, 9, 14, 18 and 22 in interphase nuclei. T- and B-lymphocytes, bone marrow and HL-60 cells were studied. The localization of the ABL and BCR genes was found at a distance of 35 - 45 % of local radius from the nuclear center, whereas c-MYC and IGH genes were found closer to the nuclear periphery. The c-MYC and IGH genes were positioned at a distance of 38 - 65% of local radius. The nuclear positions of whole chromosomes involving mentioned genes were also determined. Our results show that the topographic distributions of protooncogenes and breakpoint regions as well as those of corresponding chromosomes are basically restricted to specific nuclear regions which are largely conserved in different cell types. General conclusion is that topographic features of genetic material facilitate or impose restrictions on the formation of genetic changes. |
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