IMAGING OF ELEMENTS AND PROTEINS IN BIOLOGICAL TISSUES: MEDICAL AND PHARMACEUTICAL APPLICATIONS

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Authors

KUCHYNKA Michaela SCHEER Peter HLOŽKOVÁ Jana VLČNOVSKÁ Marcela OPATŘILOVÁ Radka KANICKÝ Viktor MASAŘÍK Michal VACULOVIČ Tomáš PADRTOVÁ Tereza

Year of publication 2022
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Pharmacy

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Description In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease [1-3]. The main emphasis of this contribution is to demonstrate the unique method of laser ablation with inductively coupled plasma and mass spectrometry (LA-ICP-MS). It is a well-established method for multi-elemental analysis of elements at trace and ultra-trace. Nowadays, it is also starting to be used as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Proteins are imaged using an immunohistochemical method (binding nanoparticle-labeled antibodies to a specific protein) and LA-ICP-MS. The main advantages of utilizing LA-ICP-MS are the acquisition of comprehensive (Metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke) or low detection limits compared to other conventionally employed protein imaging techniques in combination with multiplex analysis of one sample.
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