Detekce chlamydiaceae a chlamydia-like organismů ve všech mobilních stádiích I. ricinus
| Title in English | Detection of chlamydiaceae and chlamydia-like organisms in all mobile stages of I. ricinus |
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| Authors | |
| Year of publication | 2022 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Chlamydiaceae and chlamydia-like organisms (CLOs) are related and very large groups of bacteria found in a variety of environments and hosts. In terms of pathogenesis, they can cause a wide range of diseases, depending on the species, varying from pulmonary infections to adverse pregnancy outcomes. Recently, these organisms have been identified to a considerable extent in representatives of Ixodes ricinus and also in some skin biopsies after tick bites. It can therefore be assumed that this species of blood-sucking vector is also a carrier of these bacteria. In terms of the often-mentioned relationship of chlamydiae to the host reproductive system, it has been hypothesised that these potential pathogens may be transovarially transmitted between tick generations. This research is further divided into three chapters. In the first part, the occurrence of panchlamydial DNA in I. ricinus individuals in three parts of 2019 was mapped by Endpoint PCR. The second part was devoted to determining the percentage of panchlamydial DNA in adult ticks only. The final chapter was dedicated to the detection of panchlamydial DNA abundance in tick larvae from 15 different collections (from 2019 and 2020) and subsequent sequencing of positive samples. The first chapter showed a minimum prevalence of 30.03% and the second chapter showed 82% of positive individuals. In the final section, the minimum prevalence was 20%. Of these 27 samples, one was identified as a representative of the genus Protochlamydia, which is commonly associated with pneumonia in humans, and the others as likely unidentified representatives of Chlamydiales. These data suggest that both groups under investigation are present in ticks and there is also a very high probability of unexplored transovarial transmission. |
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