Physical and genetic map of the genome of <IStaphylococcus carnosus</I> TM300
| Title in English | Physical and genetic map of the genome of Staphylococcus carnosus TM300 |
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| Authors | |
| Year of publication | 1998 |
| Type | Article in Periodical |
| Magazine / Source | Microbiology UK |
| MU Faculty or unit | |
| Citation | |
| Web | |
| Field | Genetics and molecular biology |
| Description | A genome map of Staphylococcus carnosus TM300, an important micro- organism in the food industry and long used as a starter culture, was constructed by pulsed-field gel electrophoresis of DNA fragments obtained after digestion with NotI, SfiI and ApaI. The size of the chromosome was estimated to be 2590 kb. The fragments were assembled into a physical map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clusters were positioned on the physical map by Southern hybridization analysis. The map provides a basis for further analysis of the S. carnosus chromosome |
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