Physical and genetic map of the genome of <IStaphylococcus carnosus</I> TM300

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Title in English Physical and genetic map of the genome of Staphylococcus carnosus TM300
Authors

WAGNER Elke DOŠKAŘ Jiří GÖTZ Friedrich

Year of publication 1998
Type Article in Periodical
Magazine / Source Microbiology UK
MU Faculty or unit

Faculty of Science

Citation
Web
Field Genetics and molecular biology
Description A genome map of Staphylococcus carnosus TM300, an important micro- organism in the food industry and long used as a starter culture, was constructed by pulsed-field gel electrophoresis of DNA fragments obtained after digestion with NotI, SfiI and ApaI. The size of the chromosome was estimated to be 2590 kb. The fragments were assembled into a physical map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clusters were positioned on the physical map by Southern hybridization analysis. The map provides a basis for further analysis of the S. carnosus chromosome
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