Cyanine Phototruncation Enables Spatiotemporal Cell Labeling

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Authors

FUKUSHIMA Hiroshi MATIKONDA Siddharth S. USAMA Syed Muhammad FURUSAWA Aki KATO Takuya ŠTACKOVÁ Lenka KLÁN Petr KOBAYASHI Hisataka SCHNERMANN Martin J.

Year of publication 2022
Type Article in Periodical
Magazine / Source Journal of the American Chemical Society
MU Faculty or unit

Faculty of Science

Citation
Web https://pubs.acs.org/doi/10.1021/jacs.2c02962
Doi http://dx.doi.org/10.1021/jacs.2c02962
Keywords LYMPH-NODE; IN-VIVO; IDENTIFICATION; TRACKING; EGFP
Description Photoconvertible tracking strategies assess the dynamic migration of cell populations. Here we develop phototruncation-assisted cell tracking (PACT) and apply it to evaluate the migration of immune cells into tumor-draining lymphatics. This method is enabled by a recently discovered cyanine photoconversion reaction that leads to the two-carbon truncation and consequent blue-shift of these commonly used probes. By examining substituent effects on the heptamethine cyanine chromophore, we find that introduction of a single methoxy group increases the yield of the phototruncation reaction in neutral buffer by almost 8-fold. When converted to a membrane-bound cell-tracking variant, this probe can be applied in a series of in vitro and in vivo experiments. These include quantitative, time-dependent measurements of the migration of immune cells from tumors to tumor-draining lymph nodes. Unlike previously reported cellular photoconversion approaches, this method does not require genetic engineering and uses near-infrared (NIR) wavelengths. Overall, PACT provides a straightforward approach to label cell populations with spatiotemporal control.
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