Epitachophoretic preconcentration of proteins

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Authors	HRUŠKOVÁ Helena VORA´CˇOVÁ Ivona FORET František
Year of publication	2021
Type	Article in Proceedings
Conference	Proceedings of the 17th International Students Conference “Modern Analytical Chemistry”
MU Faculty or unit	Faculty of Science
Citation
Web	http://web.natur.cuni.cz/analchem/isc/isc17.pdf
Keywords	cleaning; epitachophoresis; isotachophoresis; preparative; concentration
Description	This project is focused on the epitachophoretic preconcentration of Proteins from biological fluids. In this paper, the method for preparative concentration of three standards of proteins, cytochrome c, myoglobin, and hemoglobin, was developer and described. Concentration conditions, such as concentration and pH of leading and trailing electrolytes, gel composition, and time of concentration, were optimized. The recovery of each protein was at least 70% and concentration factor 50.
Related projects:	Signal integration and epigenetic reprograming for plant productivity