Application-based guidelines for best practices in plant flow cytometry
| Authors | |
|---|---|
| Year of publication | 2022 |
| Type | Article in Periodical |
| Magazine / Source | Cytometry: Part A |
| MU Faculty or unit | |
| Citation | |
| Web | https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.a.24499 |
| Doi | http://dx.doi.org/10.1002/cyto.a.24499 |
| Keywords | cell cycle; DNA base composition; DNA content; endoreduplication; flow cytometric seed screening; genome size; in vitro cultures; intraspecific variation; ploidy |
| Description | Flow cytometry (FCM) is currently the most widely-used method to establish nuclearDNA content in plants. Since simple, 1-3-parameter, flow cytometers, which are suffi-cient for most plant applications, are commercially available at a reasonable price, thenumber of laboratories equipped with these instruments, and consequently newFCM users, has greatly increased over the last decade. This paper meets an urgentneed for comprehensive recommendations for best practices in FCM for differentplant science applications. We discuss advantages and limitations of establishing plantploidy, genome size, DNA base composition, cell cycle activity, and level of endo-reduplication. Applications of such measurements in plant systematics, ecology,molecular biology research, reproduction biology, tissue cultures, plant breeding, andseed sciences are described. Advice is included on how to obtain accurate and reliableresults, as well as how to manage troubleshooting that may occur during samplepreparation, cytometric measurements, and data handling. Each section is followedby best practice recommendations; tips as to what specific information should beprovided in FCM papers are also provide |
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