Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications

Investor logo

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

KOSTIV Uliana FARKA Zdeněk MICKERT Matthias Jürgen GORRIS Hans-Heiner VELYCHKIVSKA Nadiia POP-GEORGIEVSKI Ognen PASTUCHA Matěj ODSTRČILÍKOVÁ Eliška SKLÁDAL Petr HORÁK Daniel

Year of publication 2020
Type Article in Periodical
Magazine / Source Biomacromolecules
MU Faculty or unit

Faculty of Science

Citation
Web https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459
Doi http://dx.doi.org/10.1021/acs.biomac.0c00459
Keywords photon-upconversion nanoparticle; UCNP; bioconjugation; poly(ethylene glycol); click chemistry; upconversion-linked immunosorbent assay; ULISA; human serum albumin
Description Lanthanide-doped upconversion nanoparticles (UCNPs) display highly beneficial photophysical features for background-free bioimaging and bioanalysis; however, they are instable in high ionic strength buffers, have no functional groups, and are nonspecifically interacting. Here, we have prepared NIR-excitable UCNPs that are long-term colloidally stable in buffered media and possess functional groups. Heterobifunctional poly(ethylene glycol) (PEG) linkers bearing neridronate and alkyne or maleimide were attached to UCNPs via a ligand exchange. Streptavidin (SA)-conjugates were prepared by click reaction of UCNP@PEG-alkyne and SA-azide. Antihuman serum albumin pAbF antibody was modified with azide groups and conjugated to UCNP@PEG-alkyne via click reaction; alternatively, the antibody, after mild reduction of its disulfide bonds, was conjugated to UCNP@PEG-maleimide. We employed these nanoconjugates as labels for an upconversion-linked immunosorbent assay. SA-based labels achieved the lowest LOD of 0.17 ng/mL for the target albumin, which was superior compared to a fluorescence immunoassay (LOD 0.59 ng/mL) or an enzyme-linked immunoassay (LOD 0.56 ng/mL).
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info