C-peptid ovlivňuje hladinu glyoxalázy 1 v proximálních tubulárních buňkách in vitro
| Title in English | C-peptide affects the level of glyoxalase 1 in proximal tubular cells in vitro |
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| Authors | |
| Year of publication | 2020 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Introduction: Diabetic kidney disease (HDD) is the most common cause of end-stage renal disease (ESRD). The treatment strategy to stop the progression of DKD is an excellent compensation for the underlying disease, controlling blood pressure and lipids and other metabolic parameters. However, even strict adherence to treatment measures may not lead to halting progression to the ESRD. One of the protective pathways is considered to be the glyoxalase system with the enzyme glyoxalase 1 (GLO-1), the function of which is the removal of the reactive aldehyde methylglyoxal, which is formed during glycolysis and is used in the formation of DKD. Recently, the role of C-peptide in the pathogenesis of DKD has also been considered. C-peptide, which has long been considered a biologically inert molecule, has shown beneficial effects in a number of studies at both the molecular and organ levels. It increases the activity and expression of several antioxidant molecules, in animal studies and in a limited number of people has led to improved parameters of renal function. The aim of the in vitro experiment was to monitor the effect of C-peptide on GLO-1. Methods: GLO-1 expression was monitored on an immortalized epithelial cell line of the proximal tubule HK-2. Cells were cultured in basic culture medium (DMEM + Hams F-12) under four types of conditions - normoglycemia (NG), normoglycemia + C-peptide (NGC), hyperglycemia (HG) and hyperglycemia + C-peptide (HGC). The glucose concentration in the culture medium was 7.2 mmol / l and the hyperglycemia was 25 mmol / l. The C-peptide concentration was 1 nmol / l in both cases. After culturing, proteins were isolated from the cells, quantified by Western blotting and related to BETA-actin. Results: C-peptide in HG medium decreased GLO-1 expression against NG (p & lt; 0.01) and NGC (p = 0.03). The difference between HG and HGC is also noticeable, but it is not statistically significant (p = 0.06). Conclusion: C-peptide at a physiological concentration of 1 nmol / l reduces GLO-1 expression under hyperglycemic conditions in HK-2 cells. An explanation is offered that in hyperglycemia, C-peptide reduces the amount of glucose entering glycolysis or otherwise eliminates methylglyoxal. Further research is needed to answer this question. |
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