In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes
| Authors | |
|---|---|
| Year of publication | 2019 |
| Type | Article in Periodical |
| Magazine / Source | G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS |
| MU Faculty or unit | |
| Citation | |
| web | https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25 |
| Doi | http://dx.doi.org/10.1007/978-1-4939-9666-7_25 |
| Keywords | In-cell NMR; Xenopus laevis oocyte; DNA; RNA; G-quadruplex |
| Description | G-quadruplexes are inherently polymorphic nucleic acid structures. Their folding topology depends on the nucleic acid primary sequence and on physical-chemical environmental factors. Hence, it remains unclear if a G-quadruplex topology determined in the test tube (in vitro) will also form in vivo. Characterization of G-quadruplexes in their native environment has been proposed as an efficient strategy to tackle this issue. So far, characterization of G-quadruplex structures in living cells has relied exclusively on the use of Xenopus laevis oocytes as a eukaryotic cell model system. Here, we describe the protocol for the preparation of X. laevis oocytes for studies of G-quadruplexes as well as other nucleic acids motifs under native conditions using in-cell NMR spectroscopy. |
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