Využití sekvenování nové generace v analýze cirkulujících mikroRNA jako prediktivních bio markerů u pacientů s lokálně pokročilým karcinomem rekta
| Title in English | Utilization of next generation sequencing in analysis of circulating micrornas as predictive biomarkers for patients with locally advanced rectal Carcinoma |
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| Authors | |
| Year of publication | 2019 |
| Type | Article in Periodical |
| Magazine / Source | Klinická onkologie |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.prolekare.cz/casopisy/klinicka-onkologie/2019-supplementum1/vyuziti-sekvenovani-nove-generace-v-analyze-cirkulujicich-mikrorna-jako-prediktivnich-biomarkeru-u-pacientu-s-lokalne-pokrocilym-karcinomem-rekta-109557 |
| Keywords | MicroRNAs; Neoplasms; CRC patients |
| Description | MicroRNAs (miRNA) are short non-coding RNAs involved in post-transcriptional regulation of gene expression. MiRNAs are essential regulators of both physiological processes as of pathogeneses of many diseases, and their dysregulation was observed in many malignancies including rectal cancer. Circulating miRNAs presented in blood plasma could be potential candidates for non-invasive predictive bio markers of the response of patients with locally advanced rectal cancer to chemoradiotherapy. Presented study aims to evaluate the potential of next-generation sequencing in the analysis of circulating miRNAs. Material and methods: MiRNA expression profi les were done using samples of RNA isolated from blood plasma collected during TNM restaging and paired samples collected before initiation of neoadjuvant chemoradiotherapy. Sequencing libraries were prepared using kit which implements universal molecular indices that help to sensitively fi lter bio logical bias during data analysis. Sequencing data were processed by multidimensional bio statistical approaches. Conclusion: We identifi ed specific miRNA profi le enabling to distinguish the patients accordingly to their response to chemoradiotherapy. |
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