Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting

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Authors

SLAVIK Jan SKOPALIK Josef PROVAZNÍK Ivo HUBALEK Jaromir

Year of publication 2019
Type Article in Periodical
Magazine / Source Sensors
MU Faculty or unit

Faculty of Medicine

Citation
Web http://dx.doi.org/10.3390/s19245379
Doi http://dx.doi.org/10.3390/s19245379
Keywords multielectrode array; microelectrode array; sacrificial layer; peel-off; HL-1 cells; microprinting; antifouling agent
Description Multielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity.
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