Quaternary protoberberine alkaloids and their interactions with DNA

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Authors

JAROŠOVÁ Petra SÁNDOR Roman SLANINKOVÁ Andrea VIDO Marek PEŠ Ondřej TÁBORSKÝ Petr

Year of publication 2019
Type Article in Periodical
Magazine / Source Chemical Papers
MU Faculty or unit

Faculty of Science

Citation
web https://link.springer.com/article/10.1007%2Fs11696-019-00857-z
Doi http://dx.doi.org/10.1007/s11696-019-00857-z
Keywords Protoberberine alkaloids; Non-canonical structures; G-quadruplex; dsDNA; Competitive dialysis; CD; Melting temperatures; Mass spectrometry
Description The G-quadruplex DNA is commonly present in several protooncogenic-DNA promoters and participates in many important biological processes such as replication, transcription, and translation. Because of their supposed role in cancer, G-quadruplex DNA is often studied as a target for anti-cancer drugs. Quaternary protoberberine and tetrahydroprotoberberine alkaloids (corysamine, coptisine, stylopine), which are supposed to selectively bind these structures, have been compared in terms of stability with selected types of DNA. Influence of selected alkaloids on the stability of double-stranded DNA and non-canonical forms of DNA was observed by determining association constants of alkaloid-DNA complexes using spectroscopic methods-molecular absorption spectrometry, fluorescence spectrometry, and mass spectrometry. Furthermore, the effect of given alkaloids on the melting temperature of these DNA structures was determined using CD spectrometry. Competitive dialysis and electrospray mass spectrometry were performed for affinity comparison of certain alkaloids to different DNA structures including G-quadruplexes. These experiments have proven that corysamine and coptisine prefer interaction with G-quadruplexes in comparison to dsDNA and ssDNA, whereas tetrahydroprotoberberine alkaloid stylopine does not interact with any DNA whatsoever.
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