New metabolites of diclofenac found in pea and maize
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| Year of publication | 2019 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Pharmaceuticals are used widely in our society. They can be detected at trace levels in waste water. Waste water treatment technology is still not able to remove these foreign substances. Due to rising drought in Europe, usage of waste water for irrigation is increasing in many countries. Another way how pharmaceuticals enter the agriculture is to use biosolids from animal production. One of the most consumed groups of pharmaceuticals are non-steroidal anti-inflammatory drugs (NSAIDs). Diclofenac (DCF) is a member of NSAIDs. Metabolomics provide information how plants deal with stress, whether it is detection of new metabolite formation, phytohormones or change in the levels of primary metabolites. In our research we focused on detection of plant metabolites of DCF formed by maize and pea. There is information about uptake and few metabolites of DCF in Armorancia rusticana, Hordeum vulgare, Typha latifolia, Lepidum sativum and Arabidopsis thaliana. The knowledge of more metabolites is limited. Therefore our main goal was to investigate other metabolites of DCF. Our results show confirmation of formation of already known metabolites such as DCF-OH and DCF-OH-Glc. DCF metabolites having lactam moiety in the structure were found. These metabolites were previously described as 2-indolinone derivatives formed by A.thaliana. The highest concentration of all metabolites was detected in the roots in both plants. The reason might be low solubility of DCF can cause difficult transport to overground parts of plants. There are also some differences between determination of DCF metabolites using TOF and Orbitrap instrumentation. |
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