Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy

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Authors	REBROŠOVÁ Katarína ŠILER Martin SAMEK Ota RŮŽIČKA Filip BERNATOVÁ Silvie JEŽEK Jan ZEMÁNEK Pavel HOLÁ Veronika
Year of publication	2019
Type	Article in Periodical
Magazine / Source	FUTURE MICROBIOLOGY
MU Faculty or unit	Faculty of Medicine
Citation
Web	http://dx.doi.org/10.2217/fmb-2018-0297
Doi	http://dx.doi.org/10.2217/fmb-2018-0297
Keywords	biofilm; Candida parapsilosis; Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis
Description	Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: Themethod showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.
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