SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | CHEMICKÉ LISTY |
| MU Faculty or unit | |
| Citation | |
| Keywords | RECOVERY; PEPTIDES |
| Description | In the present study, we assessed peptide losses during sample storage in autosampler vials. We evaluated the possibility to suppress the adsorption effects by changing the composition of the sample solution injected into the liquid chromatography coupled with tandem mass spectrometry system (LC-MS/MS) and by use of different material of autosampler vials. As a model sample, a tryptic digest of six bovine proteins (B6E) in the amount of 1 fmol per protein was used for comparative experiments. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (5M and 1M) as injection solutions provided the best results in terms of number of significantly identified peptides (P < 0.05). These conclusions were confirmed by analyses of a real sample with intermediate complexity. Addition of PEG into the real sample solution proved to prevent higher losses, concerning mainly hydrophobic peptides, during up to 48 h storage in the autosampler in comparison with a formic acid solution and even with a solution of highly concentrated urea and thiourea(1). |
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