Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte
| Authors | |
|---|---|
| Year of publication | 2019 |
| Type | Article in Periodical |
| Magazine / Source | Chemical Papers |
| MU Faculty or unit | |
| Citation | |
| Web | https://link.springer.com/article/10.1007/s11696-018-0560-1 |
| Doi | http://dx.doi.org/10.1007/s11696-018-0560-1 |
| Keywords | Endogenous cortisol; Long-term hair concentration; Surrogate analyte LC–MS; Online SPE |
| Description | Online SPE LC–MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair. |
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