Study of metabolic activity of human embryos focused on amino acids by capillary electrophoresis with light-emitting diode-induced fluorescence detection
| Authors | |
|---|---|
| Year of publication | 2018 |
| Type | Article in Periodical |
| Magazine / Source | Electrophoresis |
| MU Faculty or unit | |
| Citation | |
| Web | http://dx.doi.org/10.1002/elps.201800265 |
| Doi | http://dx.doi.org/10.1002/elps.201800265 |
| Keywords | Amino acids; Capillary electrophoresis with light emitting diode induced fluorescence detection; |
| Description | Assisted reproduction is a quickly developing field of reproductive medicine whose importance is growing every year due to the increasing number of patients suffering from infertility. As a result, there is a need for the continuous development and/or improvement of assisted reproductive technologies. This paper presents a new method for the in vitro measurement of the amino acid turnover of developing embryos based on capillary electrophoresis with light-emitting diode-induced fluorescence detection. Amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde/NaCN, and the resulting fluorescent derivatives were baseline resolved within 25 min in a background electrolyte comprised of 50 mM sodium tetraborate, 73 mM sodium dodecyl sulphate, 5 mM sodium deoxycholate and 2.5 mM (2 hydroxypropyl)-beta cyclodextrin (pH 9.3). The migration time and the peak area repeatability (n = 10) were below 0.5 % and 4.3 %, respectively. The limits of detection ranged from 12.6 nM (histidine) to 39.3 nM (taurine). The developed method, which only requires 2 ul of raw sample, was successfully applied for determining the metabolic activity of human embryos exposed to different environmental stress conditions. |
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