Capillary electrophoresis with fluorescence detection as a tool for the study of metabolites
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| Year of publication | 2017 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Metabolomics is a scientific discipline focusing on the study of metabolome. Metabolome is the set of all metabolites in an organism. Among metabolites of great importance to living organisms belong amino acids. Their actual levels in biological fluids can reveal complex physiological state of an organism. However they exhibit low or no native fluorescence, derivatization is essential for their sensitive determination by fluorescence detection. Capillary electrophoresis offers derivatization within the capillary, where the inner space of the capillary is used at first for derivatization and then for separation. This approach can be fully automated and requires only minimum volume of sample and other reagents. This contribution deals with optimization of two methods for derivatization of amino acids with naphthalene 2,3 dicarboxaldehyde/ sodium cyanide, differing in mixing strategy of the reactants. Mixing by transverse diffusion of laminar flow profiles (TDLFP) and by electrophoretically mediated microanalysis (EMMA) were compared in terms of efficiency of the mixing, sensitivity, repeatability and applicability to various biological samples. |
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