Deregulated expression of long non-coding RNA UCA1 in multiple myeloma

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Authors

SEDLAŘÍKOVÁ Lenka GROMESOVÁ Barbora KUBACZKOVÁ Veronika RADOVÁ Lenka FILIPOVA Jana JARKOVSKÝ Jiří BROŽOVÁ Lucie VELICHOVÁ Roberta ALMÁŠI Martina PENKA Miroslav BEZDĚKOVÁ Renata ŠTORK Martin ADAM Zdeněk POUR Luděk KREJČÍ Marta KUGLÍK Petr HÁJEK Roman ŠEVČÍKOVÁ Sabina

Year of publication 2017
Type Article in Periodical
Magazine / Source European Journal of Haematology
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1111/ejh.12908
Field Oncology and hematology
Keywords biomarker; long non-coding RNA; multiple myeloma; plasma cells; prognosis; quantitative real-time PCR
Description ObjectivesLong non-coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lncRNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma (MM) patients in comparison to normal BMPCs of healthy donors (HD) in a three-phase biomarker study. MethodsExpression profile of 83 lncRNA was performed by RT2 lncRNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD. ResultsOur analysis revealed dysregulation of two lncRNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients. ConclusionOur study suggests a possible prognostic impact of UCA1 expression levels on MM patients.
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