Activity of skin fibroblast elastase was online monitored by capillary electrophoresis
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| Year of publication | 2017 |
| Type | Conference abstract |
| Citation | |
| Description | Skin fibroblast elastase (SFE) is one of the important enzymes involved in skin aging. It participates on the degradation of the elastic fibers which form a network that contributes to the elasticity of skin and their decreasing results in wrinkle formation. We examined activity of commercially available purified enzyme and cells lysate prepared from human normal fibroblasts and keratinocytes by capillary electrophoresis using in-capillary nanoreactor based on mixing by transverse diffusion of laminar flow profiles. In this study the 5-carboxyfluorescein labeled peptides were used as substrates. Their SFE cleavage resulted in fluorescently labeled products, which enabled to employ very specific and also sensitive LIF detection. All incubations in this study were performed by online nanoreactor to minimize reactant consumption. |
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