The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

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Authors

MANAKHOV Anton MAKHNEVA Ekaterina SKLÁDAL Petr NEČAS David CECHAL Jan KALINA Lukas ELIÁŠ Marek ZAJÍČKOVÁ Lenka

Year of publication 2016
Type Article in Periodical
Magazine / Source Applied Surface Science
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.sciencedirect.com/science/article/pii/S0169433215025970
Doi http://dx.doi.org/10.1016/j.apsusc.2015.10.178
Field Physical chemistry and theoretical chemistry
Keywords Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM
Description The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiolbased self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications. (C) 2015 Elsevier B.V. All rights reserved.
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