Sensitive determination of SEVNL and DAEFR pentapeptides as beta-secretase products by CE-C4D for its activity assay
| Authors | |
|---|---|
| Year of publication | 2016 |
| Type | Article in Proceedings |
| Conference | 13th International Interdisciplinary Meeting on Bioanalysis (CECE2016) |
| MU Faculty or unit | |
| Citation | |
| web | http://www.ce-ce.org |
| Field | Biochemistry |
| Keywords | beta-secretase; Alzheimer desease; CE; C4D |
| Attached files | |
| Description | Alzheimer’s disease is the most common form of dementia; however, effective treatment is not yet available. Mechanism of the disease involves formation of neurotoxic oligomers of beta-amyloid peptides. These peptides are produced mainly by beta-secretase thus inhibition of the enzyme might be a way to treat the disease. In order to study enzyme’s inhibition – preferably with unlabeled substrates – high method’s sensitivity is demanded. Consequently, highly sensitive capillary electrophoresis based method with contactless conductivity detection was developed. |
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